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rig i reporter cell line  (InvivoGen)


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    InvivoGen rig i reporter cell line
    Rig I Reporter Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rig+i+reporter+cell+line/HEK-Lucia+RIG-I+Cells/pm41603157-242-1-7
    Average 94 stars, based on 18 article reviews
    rig i reporter cell line - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Activity Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Transfection:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Activation Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).



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    Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in <t>HEK293T</t> cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.
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    Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in <t>HEK293T</t> cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.
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    Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in <t>HEK293T</t> cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.
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    Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in <t>HEK293T</t> cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.
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    Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.

    Journal: Scientific reports

    Article Title: TSC complex decrease the expression of mTOR by regulated miR-199b-3p.

    doi: 10.1038/s41598-025-85706-8

    Figure Lengend Snippet: Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.

    Article Snippet: HEK293T cells were maintained in DMEM (Boster) supplemented with 10% FBS (BI).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Sequencing, Luciferase, Reporter Assay, Control

    Fig. 5. TSC complex regulates the endogenous content of miR199b-3p, *p < 0.05, **p < 0.01,***p < 0.001 comparison with WT. (A) The efficiency of knockdown TSC1 in shTSC1-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (B) The expression of miR-199b-3p in shTSC1-HEK293T cells and overexpression TSC1-WT or TSC1-N837fs in HEK293T cell line. (C) Quantitative analysis of mTOR in shTSC1-HEK293T cell line and overexpression TSC1-WT or TSC1 N837fs in HEK293T cell line using western blot (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S6. (D) The efficiency of knockdown TSC2 in shTSC2-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (E) The expression of miR-199b-3p in shTSC2-HEK293T cell line and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line. (F) Quantitative analysis of mTOR in shTSC2-HEK293T cell line (low panel) and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line using Western blot (low panel) and the statistics analysis (up panel). The originals gels can be seen in the supplementary figure S6.

    Journal: Scientific reports

    Article Title: TSC complex decrease the expression of mTOR by regulated miR-199b-3p.

    doi: 10.1038/s41598-025-85706-8

    Figure Lengend Snippet: Fig. 5. TSC complex regulates the endogenous content of miR199b-3p, *p < 0.05, **p < 0.01,***p < 0.001 comparison with WT. (A) The efficiency of knockdown TSC1 in shTSC1-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (B) The expression of miR-199b-3p in shTSC1-HEK293T cells and overexpression TSC1-WT or TSC1-N837fs in HEK293T cell line. (C) Quantitative analysis of mTOR in shTSC1-HEK293T cell line and overexpression TSC1-WT or TSC1 N837fs in HEK293T cell line using western blot (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S6. (D) The efficiency of knockdown TSC2 in shTSC2-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (E) The expression of miR-199b-3p in shTSC2-HEK293T cell line and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line. (F) Quantitative analysis of mTOR in shTSC2-HEK293T cell line (low panel) and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line using Western blot (low panel) and the statistics analysis (up panel). The originals gels can be seen in the supplementary figure S6.

    Article Snippet: HEK293T cells were maintained in DMEM (Boster) supplemented with 10% FBS (BI).

    Techniques: Comparison, Knockdown, Expressing, Over Expression, Western Blot